Hand hygiene is crucial in preventing healthcare-associated infections. Despite global efforts, hospital-acquired infections persist, and hand hygiene compliance remains low, averaging around 40%. While alcohol-based hand rubs (ABHR) effectively prevent infection spread, improper glove use and non-compliance with the World Health Organization's five moments for hand hygiene are common challenges in healthcare settings. A key barrier to compliance is the high workload of healthcare workers, who often lack time to remove gloves and disinfect hands between patient interactions. In situations where glove changes are impractical, glove disinfection may offer a feasible alternative. Evidence suggests that disinfecting gloves with ABHR could reduce contamination and improve compliance. With limited personal protective equipment resources in hospital settings, glove disinfection has shown potential benefits, including reduced workload, cost savings, and environmental impact. Studies demonstrate that regular glove disinfection significantly reduces microbial transmission compared to the gold standard of hand hygiene. Despite these findings, further research is necessary to assess the long-term safety and effectiveness of glove disinfection before it can be widely adopted as an alternative to glove removal and hand hygiene.